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https://github.com/Polarolouis/anova-phylogenetique-projet-msv.git
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9.8 KiB
Text
255 lines
No EOL
9.8 KiB
Text
Ici nous appliquons les méthodes implémentées sur l'arbre de \cite{chenQuantitativeFrameworkCharacterizing2019}.
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<< 'knitr_options', echo = FALSE>>=
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knitr::opts_knit$set(cache = TRUE)
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@
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<< import_modules, echo = FALSE, include=FALSE>>=
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necessary_packages <- c("phylotools", "phytools", "phylolm", "limma", "edgeR",
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"here", "ggplot2", "patchwork", "dplyr", "tidyr", "UpSetR", "evemodel",
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"compcodeR", "mvSLOUCH")
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if (!all(necessary_packages %in% installed.packages())) {
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install.packages(necessary_packages)
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install.packages("remotes")
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remotes::install_gitlab("sandve-lab/evemodel")
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remotes::install_github("pbastide/compcodeR", ref = "phylolimma")
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} else {
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require(phylotools)
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require(phytools)
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require(phylolm)
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require(limma)
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require(edgeR)
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require(here)
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require(ggplot2)
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require(dplyr)
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require(tidyr)
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require(UpSetR)
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require(evemodel)
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require(compcodeR)
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require(mvSLOUCH)
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require(patchwork)
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}
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source(here("R","utils.R"))
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@
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<< import_donnees, echo = FALSE>>=
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### Data import
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cdata <- readRDS(here("data", "data_TER", "data", "chen2019_rodents_cpd.rds"))
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is.valid <- compcodeR:::check_phyloCompData(cdata)
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if (!(is.valid == TRUE)) stop("Not a valid phyloCompData object.")
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# Design
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design_formula <- as.formula(~condition)
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design_data <- compcodeR:::sample.annotations(cdata)[, "condition", drop = FALSE]
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design_data$condition <- factor(design_data$condition)
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design <- model.matrix(design_formula, design_data)
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# Normalisation
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nf <- edgeR::calcNormFactors(compcodeR:::count.matrix(cdata) / compcodeR:::length.matrix(cdata), method = "TMM")
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lib.size <- colSums(compcodeR:::count.matrix(cdata) / compcodeR:::length.matrix(cdata)) * nf
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data.norm <- sweep((compcodeR:::count.matrix(cdata) + 0.5) / compcodeR:::length.matrix(cdata), 2, lib.size + 1, "/")
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data.norm <- data.norm * 1e6
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# Transformation
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data.trans <- log2(data.norm)
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rownames(data.trans) <- rownames(compcodeR:::count.matrix(cdata))
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@
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\subsection*{Vanilla (ML et REML), Satterthwaite (ML et REML), LRT}
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<< calcul_pvaleurs, echo = FALSE>>=
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### Pvalues computation
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pvalues_data = "chen2019pvalues.Rds"
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if (!file.exists(here("data",pvalues_data))){
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# computing pvalues vec for all genes
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pvalue_vec_vanilla <- sapply(seq(1, nrow(data.trans)), function(row_id) {
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trait <- data.trans[row_id, ]
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fit_phylo <- phylolm(trait ~ design_data$condition, phy = cdata@tree, measurement_error = TRUE)
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compute_vanilla_pvalue(fit_phylo)
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})
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pvalue_vec_vanilla <- setNames(pvalue_vec_vanilla, rownames(data.trans))
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pvalue_vec_vanilla_adj <- p.adjust(pvalue_vec_vanilla, method = "BH")
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pvalue_vec_vanilla.REML <- sapply(seq(1, nrow(data.trans)), function(row_id) {
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trait <- data.trans[row_id, ]
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fit_phylo <- phylolm(trait ~ design_data$condition, phy = cdata@tree, REML = TRUE, measurement_error = TRUE)
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compute_vanilla_pvalue(fit_phylo)
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})
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pvalue_vec_vanilla.REML <- setNames(pvalue_vec_vanilla.REML, rownames(data.trans))
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pvalue_vec_vanilla_adj.REML <- p.adjust(pvalue_vec_vanilla.REML, method = "BH")
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pvalue_vec_satterthwaite <- sapply(seq(1, nrow(data.trans)), function(row_id) {
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trait <- data.trans[row_id, ]
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fit_phylo <- phylolm(trait ~ design_data$condition, phy = cdata@tree, measurement_error = TRUE)
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compute_satterthwaite_pvalue(fit_phylo, tree = cdata@tree)
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})
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pvalue_vec_satterthwaite <- setNames(pvalue_vec_satterthwaite, rownames(data.trans))
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pvalue_vec_satterthwaite_adj <- p.adjust(pvalue_vec_satterthwaite, method = "BH")
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pvalue_vec_lrt <- sapply(seq(1, nrow(data.trans)), function(row_id) {
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trait <- data.trans[row_id, ]
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fit_phylo <- phylolm(trait ~ design_data$condition, phy = cdata@tree, measurement_error = TRUE)
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compute_lrt_pvalue(fit_phylo, tree = cdata@tree)
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})
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pvalue_vec_lrt <- setNames(pvalue_vec_lrt, rownames(data.trans))
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pvalue_vec_lrt_adj <- p.adjust(pvalue_vec_lrt, method = "BH")
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# REML
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pvalue_vec_satterthwaite.REML <- sapply(seq(1, nrow(data.trans)), function(row_id) {
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trait <- data.trans[row_id, ]
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fit_phylo <- phylolm(trait ~ design_data$condition, phy = cdata@tree, REML = TRUE, measurement_error = TRUE)
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compute_satterthwaite_pvalue(fit_phylo, tree = cdata@tree, REML = TRUE)
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})
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pvalue_vec_satterthwaite.REML <- setNames(pvalue_vec_satterthwaite.REML, rownames(data.trans))
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pvalue_vec_satterthwaite_adj.REML <- p.adjust(pvalue_vec_satterthwaite.REML, method = "BH")
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## Préparation du dataframe
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pvalues_dataframe <- data.frame(
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gene = rep(rownames(data.trans), 5),
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pvalue = c(pvalue_vec_vanilla_adj,
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pvalue_vec_vanilla_adj.REML,
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pvalue_vec_satterthwaite_adj,
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pvalue_vec_lrt_adj,
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pvalue_vec_satterthwaite_adj.REML),
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test_method = rep(c( "VanillaAdj", "VanillaAdjREML",
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"SatterthwaiteAdj", "LRTAdj",
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"SatterthwaiteAdjREML"), each = nrow(data.trans))
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)
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pvalues_dataframe$test_method <- as.factor(pvalues_dataframe$test_method)
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pvalues_dataframe <- pvalues_dataframe %>% mutate(selected = ifelse(pvalue < 0.05, 1, 0))
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save(pvalues_dataframe, file = here("data", pvalues_data))
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} else {
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load(here("data", pvalues_data))
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}
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@
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\begin{figure}
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<< graphique_all_pvalues, echo = FALSE>>=
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all_plots <- plot_spacer()
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for (test in unique(pvalues_dataframe$test_method)) {
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all_plots <- all_plots + ggplot(pvalues_dataframe %>% filter(test_method == test)) +
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aes(x = reorder(gene, -pvalue),
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y = pvalue, color = as.factor(selected)) +
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labs(color = "Selected", x = "Genes", y = "P-values")+
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# geom_bar(stat = "identity", position = "dodge") +
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geom_point()+
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geom_hline(yintercept = 0.05) +
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facet_grid(cols = vars(test_method)) +
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theme(axis.text.x=element_blank(), axis.ticks.x = element_blank())
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}
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all_plots + patchwork::plot_layout(guides = "collect",
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axis_titles = "collect", tag_level = "new") +
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plot_annotation(title = "Selected genes by tested methods")
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@
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\end{figure}
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Ici on réalise un \texttt{pivot wider} pour montrer les gènes sélectionnées par méthodes.
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<< wide_data, echo = FALSE>>=
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pvalues_dataframe_wide <- pvalues_dataframe[pvalues_dataframe$Adj,] %>%
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pivot_wider(id_cols = gene,
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names_from = test_method,
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values_from = selected) %>%
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data.frame()
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@
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\begin{figure}
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<< upset_selection, echo = FALSE>>=
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upset(pvalues_dataframe_wide,
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nsets = 5,
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mainbar.y.label = "Nombre de gènes en commun",
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sets.x.label = "Nombre de gènes sélectionnés")
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@
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\end{figure}
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\subsection*{EVEmodel}
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<<'evemodel', echo = FALSE>>=
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eve_data = "evechen2019pvalues.Rds"
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if (!file.exists(here("data", eve_data))){
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# TODO comparer avec le package evemodel, twothetatest
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# Comparer avec OU lrt
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# Arbre sans les replicats et les genes data
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# remotes::install_gitlab("sandve-lab/evemodel")
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# TODO Utiliser les infos de la ligne 83 du Rmd
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cdataEVE <- readRDS(here("data", "data_TER", "data", "chen2019_rodents_cpd.rds"))
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is.valid <- compcodeR:::check_phyloCompData(cdataEVE)
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if (!(is.valid == TRUE)) stop('Not a valid phyloCompData object.')
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tree_rep <- compcodeR:::getTree(cdataEVE)
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tree_norep <- compcodeR:::getTreeEVE(cdataEVE)
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theta_2_vec <- compcodeR:::getIsTheta2edge(cdataEVE, tree_norep)
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#col_species <- tree_norep$tip.label[compcodeR:::sample.annotations(cdataEVE)$id.species]
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col_species <- tree_norep$tip.label[cumsum(!duplicated(compcodeR:::sample.annotations(cdataEVE)$id.species))]
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# Normalisation
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nfEVE <- edgeR::calcNormFactors(compcodeR:::count.matrix(cdataEVE) / compcodeR:::length.matrix(cdataEVE), method = 'TMM')
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lib.sizeEVE <- colSums(compcodeR:::count.matrix(cdataEVE) / compcodeR:::length.matrix(cdataEVE)) * nfEVE
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data.normEVE <- sweep((compcodeR:::count.matrix(cdataEVE) + 0.5) / compcodeR:::length.matrix(cdataEVE), 2, lib.sizeEVE + 1, '/')
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data.normEVE <- data.normEVE * 1e6
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# Transformation
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data.transEVE <- log2(data.normEVE)
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rownames(data.transEVE) <- rownames(compcodeR:::count.matrix(cdataEVE))
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# Analysis with EVE
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evemodel.results_list <- evemodel::twoThetaTest(tree = tree_norep, gene.data = data.transEVE, isTheta2edge = theta_2_vec, colSpecies = col_species, upperBound = c(theta = Inf, sigma2 = Inf, alpha = log(2)/0.001/1))
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result.table <- data.frame(pvalue = pchisq(evemodel.results_list$LRT, df = 1, lower.tail = FALSE), logFC = compcodeR:::getlogFCEVE(evemodel.results_list$twoThetaRes, theta_2_vec, tree_norep))
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result.table$score <- 1 - result.table$pvalue
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result.table$adjpvalue <- p.adjust(result.table$pvalue, 'BH')
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rownames(result.table) <- rownames(compcodeR:::count.matrix(cdataEVE))
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evemodel_dataframe <- data.frame(gene = rep(rownames(result.table)),
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pvalue = c(result.table$adjpvalue),
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test_method = rep("EVEAdj", each = nrow(result.table)))
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save(evemodel_dataframe, file = here("data", eve_data))
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} else {
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load(file = here("data", eve_data))
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}
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evegenesNA <- (evemodel_dataframe%>% filter(is.na(pvalue)))$gene
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evemodel_dataframe <- evemodel_dataframe %>%
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filter(!is.na(pvalue)) %>%
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mutate(selected = ifelse(pvalue < 0.05, 1, 0))
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evemodel_dataframe$test_method <- as.factor(evemodel_dataframe$test_method)
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@
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<<'na-genes', result = 'asis'>>=
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cat(paste("Il y a eu des NAs pour les gènes : ", paste0(evegenesNA, collapse = ";")))
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@
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\subsection{Toutes les méthodes}
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<< pvalue_eve_upset, echo = FALSE>>=
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pvalueseve_dataframe <- rbind(pvalues_dataframe, evemodel_dataframe)
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pvalueseve_dataframe_wide <- pvalueseve_dataframe %>%
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pivot_wider(id_cols = gene,
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names_from = test_method,
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values_from = selected, values_fill = 0) %>%
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data.frame()
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upset(pvalueseve_dataframe_wide,
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nsets = 10,
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mainbar.y.label = "Nombre de gènes en commun",
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sets.x.label = "Nombre de gènes sélectionnés")
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@ |